The KO Validated RhoC Polyclonal Antibody (CAB1062) is a key tool for researchers studying RhoC, a protein involved in cell migration and invasion, particularly in the context of cancer metastasis. This antibody, produced in rabbits, has been extensively validated for use in Western blot applications and is highly specific for detecting RhoC in human samples.RhoC is a member of the Rho GTPase family and is known to regulate the actin cytoskeleton, cell adhesion, and cell motility. Overexpression of RhoC has been linked to increased invasiveness and metastatic potential in various cancer types, making it a promising target for therapeutic intervention.
The KO Validated RhoC Polyclonal Antibody allows for precise and reliable detection of RhoC levels in cell lines and tissues, enabling researchers to further explore its role in cancer progression and potentially identify new treatment strategies. Overall, this antibody is an essential tool for studying the complex mechanisms of cancer metastasis and may lead to the development of novel therapeutic approaches aimed at inhibiting RhoC-mediated processes in cancer cells.
Product Name:
[KO Validated] RhoC Rabbit Polyclonal Antibody
SKU:
CAB1062
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human RhoC (NP_786886.1).
This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Purification Method:
Affinity purification
Gene ID:
389
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts from normal (control) and RhoC knockout (KO) 293T cells, using RhoC antibody (CAB1062) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 300s.
