The RAD23B Polyclonal Antibody (CAB1034) is a valuable tool for researchers studying the RAD23B protein, a key player in DNA repair and proteasomal degradation pathways. This antibody, raised in rabbits, shows high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to RAD23B, researchers can detect and analyze this important protein in a variety of cell types, making it an essential tool for studies in molecular biology and cancer research.
RAD23B is a crucial component of the DNA repair system, aiding in the recognition and removal of damaged DNA. Dysregulation of RAD23B has been linked to genomic instability and cancer development, highlighting the importance of studying its function in disease contexts. The RAD23B Polyclonal Antibody provides researchers with a reliable means of investigating the role of RAD23B in DNA repair processes and its potential implications for cancer therapy and drug development.
Product Name:
Rad23B Rabbit Polyclonal Antibody
SKU:
CAB1034
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Rad23B (NP_002865.1).
Sequence:
TSST TTTV AQAP TPVP ALAP TSTP ASIT PASA TASS EPAP ASAA KQEK PAEK PAET PVAT SPTA TDST SGDS SRSN LFED ATSA LVTG QSYE NMVT EIMS M
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
P58; HR23B; HHR23B; Rad23B
Positive Sample:
SK-OV-3
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
43kDa
Observed MW:
58kDa
The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Purification Method:
Affinity purification
Gene ID:
5887
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from SK-OV-3 cells using Rad23B Rabbit pAb(CAB1034) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
