Anti-PYGB Antibody (CAB19910)[KO Validated]
- SKU:
- CAB19910
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Description
Anti-PYGB Antibody (CAB19910)[KO Validated]
The KO Validated PYGB Rabbit Polyclonal Antibody (CAB19910) is a powerful tool for researchers studying PYGB, a key enzyme involved in glycogen metabolism. This antibody, generated in rabbits, demonstrates high specificity and sensitivity for PYGB in various biological samples, making it ideal for Western blot applications.PYGB, also known as brain glycogen phosphorylase, plays a crucial role in breaking down glycogen into glucose for energy production in the brain and muscle tissues. Dysregulation of PYGB activity has been linked to metabolic disorders, neurodegenerative diseases, and cancer, highlighting the importance of studying this enzyme in different physiological and pathological conditions.
By utilizing the KO Validated PYGB Rabbit Polyclonal Antibody, researchers can accurately detect and quantify PYGB levels, aiding in the investigation of glycogen metabolism and its implications in various health conditions. This antibody is a valuable asset for studies in biochemistry, cell biology, and metabolic research, providing insights into the molecular mechanisms underlying glycogen metabolism and its impact on human health.
| Antibody Name: | Anti-PYGB Antibody [KO Validated] |
| Antibody SKU: | CAB19910 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of human PYGB. |
| Application: | WB IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:20 - 1:100 IP 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa |
| Immunogen: | Recombinant protein of human PYGB. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 5834 |
| Uniprot: | P11216 |
| Cellular Location: | |
| Calculated MW: | 96kDa |
| Observed MW: | 97KDa |
![Anti-PYGB Antibody [KO Validated] (CAB19910)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/q/331/A19910_1__83644.jpg) | Western blot analysis of extracts from normal (control) and PYGB knockout (KO) HeLa cells, using PYGB antibody (CAB19910) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
| Synonyms: | PYGB, GPBB |
| Background: | The protein encoded by this gene is a glycogen phosphorylase found predominantly in the brain. The encoded protein forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is positively regulated by AMP and negatively regulated by ATP, ADP, and glucose-6-phosphate. This enzyme catalyzes the rate-determining step in glycogen degradation. |
| UniProt Protein Function: | PYGB: Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Belongs to the glycogen phosphorylase family. |
| UniProt Protein Details: | Protein type:Phosphorylase; Kinase, other; Carbohydrate Metabolism - starch and sucrose; Transferase; EC 2.4.1.1 Chromosomal Location of Human Ortholog: 20p11.21 Cellular Component: cytoplasm; membrane Molecular Function:glycogen phosphorylase activity; protein binding; pyridoxal phosphate binding Biological Process: glycogen catabolic process |
| NCBI Summary: | The protein encoded by this gene is a glycogen phosphorylase found predominantly in the brain. The encoded protein forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is positively regulated by AMP and negatively regulated by ATP, ADP, and glucose-6-phosphate. This enzyme catalyzes the rate-determining step in glycogen degradation. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P11216 |
| NCBI GenInfo Identifier: | 20178317 |
| NCBI Gene ID: | 5834 |
| NCBI Accession: | P11216.5 |
| UniProt Secondary Accession: | P11216,Q96AK1, Q9NPX8, |
| UniProt Related Accession: | P11216 |
| Molecular Weight: | 96,696 Da |
| NCBI Full Name: | Glycogen phosphorylase, brain form |
| NCBI Synonym Full Names: | phosphorylase, glycogen; brain |
| NCBI Official Symbol: | PYGBÂ Â |
| NCBI Official Synonym Symbols: | GPBBÂ Â |
| NCBI Protein Information: | glycogen phosphorylase, brain form |
| UniProt Protein Name: | Glycogen phosphorylase, brain form |
| Protein Family: | Glycogen phosphorylase |
| UniProt Gene Name: | PYGBÂ Â |
| UniProt Entry Name: | PYGB_HUMAN |
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