The PTPN11 Polyclonal Antibody (CAB12486) is a valuable tool for research focused on PTPN11, a protein tyrosine phosphatase involved in various cellular signaling pathways. This antibody, produced in rabbits, targets PTPN11 and is validated for use in Western blot applications with human samples. By specifically binding to PTPN11, researchers can detect and analyze this critical protein in different cell types, making it suitable for studies in cell signaling, cancer biology, and developmental biology.
PTPN11, also known as SHP2, is a key player in multiple signaling cascades, including growth factor and cytokine pathways. Dysregulation of PTPN11 has been implicated in various diseases, making it a promising target for therapeutic intervention. By understanding the role of PTPN11 in different cellular processes, researchers can uncover potential targets for drug development and treatment strategies for diseases such as cancer, developmental disorders, and cardiovascular diseases.
Product Name:
[KO Validated] SHP2 Rabbit Polyclonal Antibody
SKU:
CAB12486
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-460 of human SHP2 (NP_002825.3).
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia.
Purification Method:
Affinity purification
Gene ID:
5781
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and SHP2 knockout (KO) 293T cells, using [KO Validated] SHP2 Rabbit pAb (CAB12486) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
