The PTBP1 Polyclonal Antibody (CAB18052) is a valuable tool for researchers studying PTBP1, a key regulator of mRNA splicing and stability. This antibody, produced in rabbits, exhibits high reactivity with PTBP1 in human samples and is validated for use in Western blot applications. By binding to the PTBP1 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an essential component in studies focusing on RNA biology and gene expression regulation.PTBP1, also known as polypyrimidine tract-binding protein 1, plays a crucial role in post-transcriptional gene regulation by controlling the splicing and translation of mRNA molecules.
Its involvement in processes such as alternative splicing and mRNA stability makes it a promising target for research related to diseases like cancer, neurodegenerative disorders, and viral infections. Investigating the function of PTBP1 is essential for gaining insights into the mechanisms underlying these conditions and developing targeted therapies.
Product Name:
[KO Validated] PTBP1 Rabbit Polyclonal Antibody
SKU:
CAB18052
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human PTBP1 (NP_114368.1).
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described.
Purification Method:
Affinity purification
Gene ID:
5725
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and PTBP1 knockout (KO) HeLa cells, using [KO Validated] PTBP1 Rabbit pAb (CAB18052) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
