The PSME3 Polyclonal Antibody (CAB18021) is a valuable tool for researchers studying PSME3, a key protein involved in the regulation of immune responses and the degradation of proteins within the cell. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.PSME3, also known as proteasome activator complex subunit 3, plays a crucial role in the activation of the proteasome, a complex responsible for degrading unwanted or damaged proteins in the cell.
Dysregulation of PSME3 has been implicated in various diseases, including cancer, autoimmune disorders, and neurodegenerative conditions.By using the PSME3 Polyclonal Antibody, researchers can accurately detect and analyze the expression of PSME3 in different cell types, providing insight into its function and potential therapeutic targets. This antibody is a valuable tool for studies in immunology, cancer research, and other areas where protein degradation and immune regulation play a role.
Product Name:
[KO Validated] PSME3 Rabbit Polyclonal Antibody
SKU:
CAB18021
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 200-254 of human PSME3 (NP_005780.2).
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring. Alternate splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
10197
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and PSME3 knockout (KO) 293T cells, using [KO Validated] PSME3 Rabbit pAb (CAB18021) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
