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[KO Validated] PSME2 Rabbit Polyclonal Antibody (CAB19864)

SKU:
CAB19864
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
$167 - $503
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KO Validated] PSME2 Rabbit Polyclonal Antibody
SKU:CAB19864
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-239 of human PSME2 (NP_002809.2).
Sequence:MAKP CGVR LSGE ARKQ VEVF RQNL FQEA EEFL YRFL PQKI IYLN QLLQ EDSL NVAD LTSL RAPL DIPI PDPP PKDD EMET DKQE KKEV HKCG FLPG NEKV LSLL ALVK PEVW TLKE KCIL VITW IQHL IPKI EDGN DFGV AIQE KVLE RVNA VKTK VEAF QTTI SKYF SERG DAVA KASK ETHV MDYR ALVH ERDE AAYG ELRA MVLD LRAF YAEL YHII SSNL EKIV NPKG EEKP SMY
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:PA28B; REGbeta; PA28beta; E2
Positive Sample:293T
Conjugate:Unconjugated
Cellular Localization:cytoplasm, cytosol, extracellular exosome, nucleoplasm
Calculated MW:27kDa
Observed MW:27kDa

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the beta subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three beta and three alpha subunits combine to form a heterohexameric ring. Six pseudogenes have been identified on chromosomes 4, 5, 8, 10 and 13.

Purification Method:Affinity purification
Gene ID:5721
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from wild type (WT) and PSME2 knockout (KO) 293T cells, using [KO Validated] PSME2 Rabbit pAb (CAB19864) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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