The PSME2 Polyclonal Antibody (CAB19864) is a valuable tool for researchers studying the PSME2 protein, an essential component of the immunoproteasome complex. This rabbit-derived antibody is highly specific to human samples and has been validated for use in Western blot applications, allowing for reliable detection and analysis of PSME2 in various cell types.PSME2, also known as PA28β, is involved in the regulation of immune responses by facilitating antigen processing and presentation. Its role in the immunoproteasome complex makes it a key player in the adaptive immune system, with implications for autoimmune diseases, viral infections, and cancer.
By targeting PSME2 with this antibody, researchers can gain deeper insights into the mechanisms of immune regulation and potentially develop new therapies for immune-related conditions.Overall, the PSME2 Polyclonal Antibody (CAB19864) is a valuable tool for investigating the role of PSME2 in immune function and disease, offering researchers a reliable and specific reagent for their studies in immunology and cancer research.
Product Name:
[KO Validated] PSME2 Rabbit Polyclonal Antibody
SKU:
CAB19864
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-239 of human PSME2 (NP_002809.2).
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the beta subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three beta and three alpha subunits combine to form a heterohexameric ring. Six pseudogenes have been identified on chromosomes 4, 5, 8, 10 and 13.
Purification Method:
Affinity purification
Gene ID:
5721
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and PSME2 knockout (KO) 293T cells, using [KO Validated] PSME2 Rabbit pAb (CAB19864) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
