The PSMB8 Polyclonal Antibody (CAB19933) is a valuable tool for researchers studying the proteasome subunit beta type-8 (PSMB8) protein. This antibody is raised in rabbits and is validated for use in various applications, including Western blotting. It specifically binds to the PSMB8 protein, allowing for detection and analysis in different cell types.PSMB8 is a key component of the proteasome complex, which plays a crucial role in protein degradation and antigen presentation. Dysregulation of the proteasome system has been implicated in various diseases, including autoimmune disorders and cancer.
By targeting PSMB8, researchers can gain insights into the mechanisms underlying these diseases and develop potential therapies.The PSMB8 Polyclonal Antibody is an essential tool for studying the function and regulation of PSMB8 in various biological contexts. Its high reactivity with human samples makes it an ideal choice for researchers in the fields of immunology, cancer research, and autoimmune diseases.
Product Name:
[KO Validated] PSMB8 Rabbit Polyclonal Antibody
SKU:
CAB19933
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 163-272 of human PSMB8 (NP_004150.1).
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit.
Purification Method:
Affinity purification
Gene ID:
5696
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and PSMB8 knockout (KO) A-549 cells, using [KO Validated] PSMB8 Rabbit pAb (CAB19933) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1min.
