Anti-PARK7 Antibody (CAB0987)

Antibody Name:	Anti-PARK7 Antibody [KO Validated]
Antibody SKU:	CAB0987
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-189 of human PARK7 (NP_001116849.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HeLa, NIH/3T3, Jurkat

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-189 of human PARK7 (NP_001116849.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MASK RALV ILAK GAEE METV IPVD VMRR AGIK VTVA GLAG KDPV QCSR DVVI CPDA SLED AKKE GPYD VVVL PGGN LGAQ NLSE SAAV KEIL KEQE NRKG LIAA ICAG PTAL LAHE IGFG SKVT THPL AKDK MMNG GHYT YSEN RVEK DGLI LTSR GPGT SFEF ALAI VEAL NGKE VAAQ VKAP LVLK D
Gene ID:	11315
Uniprot:	Q99497
Cellular Location:	Cell membrane, Cytoplasm, Lipid-anchor, Membrane raft, Mitochondrion, Nucleus
Calculated MW:	19kDa
Observed MW:	20kDa

Synonyms:

DJ-1, DJ1, GATD2, HEL-S-67p, PARK7

Background:

The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene.

UniProt Protein Function:	Protein and nucleotide deglycase that catalyzes the deglycation of the Maillard adducts formed between amino groups of proteins or nucleotides and reactive carbonyl groups of glyoxals (PubMed:25416785, PubMed:28596309). Thus, functions as a protein deglycase that repairs methylglyoxal- and glyoxal-glycated proteins, and releases repaired proteins and lactate or glycolate, respectively. Deglycates cysteine, arginine and lysine residues in proteins, and thus reactivates these proteins by reversing glycation by glyoxals. Acts on early glycation intermediates (hemithioacetals and aminocarbinols), preventing the formation of advanced glycation endproducts (AGE) that cause irreversible damage (PubMed:25416785, PubMed:28013050, PubMed:26995087). Also functions as a nucleotide deglycase able to repair glycated guanine in the free nucleotide pool (GTP, GDP, GMP, dGTP) and in DNA and RNA. Is thus involved in a major nucleotide repair system named guanine glycation repair (GG repair), dedicated to reversing methylglyoxal and glyoxal damage via nucleotide sanitization and direct nucleic acid repair (PubMed:28596309). Also displays an apparent glyoxalase activity that in fact reflects its deglycase activity (PubMed:22523093). Plays an important role in cell protection against oxidative stress and cell death acting as oxidative stress sensor and redox-sensitive chaperone and protease; functions probably related to its primary function (PubMed:17015834, PubMed:20304780, PubMed:18711745, PubMed:12796482, PubMed:19229105, PubMed:25416785, PubMed:26995087). It is involved in neuroprotective mechanisms like the stabilization of NFE2L2 and PINK1 proteins, male fertility as a positive regulator of androgen signaling pathway as well as cell growth and transformation through, for instance, the modulation of NF-kappa-B signaling pathway (PubMed:12612053, PubMed:15502874, PubMed:14749723, PubMed:17015834, PubMed:21097510, PubMed:18711745). Eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death (PubMed:16390825). Required for correct mitochondrial morphology and function as well as for autophagy of dysfunctional mitochondria (PubMed:19229105, PubMed:16632486). Plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking (PubMed:18711745). Regulates astrocyte inflammatory responses, may modulate lipid rafts-dependent endocytosis in astrocytes and neuronal cells (PubMed:23847046). In pancreatic islets, involved in the maintenance of mitochondrial reactive oxygen species (ROS) levels and glucose homeostasis in an age- and diet dependent manner. Protects pancreatic beta cells from cell death induced by inflammatory and cytotoxic setting. Binds to a number of mRNAs containing multiple copies of GG or CC motifs and partially inhibits their translation but dissociates following oxidative stress (PubMed:18626009). Metal-binding protein able to bind copper as well as toxic mercury ions, enhances the cell protection mechanism against induced metal toxicity (PubMed:23792957). In macrophages, interacts with the NADPH oxidase subunit NCF1 to direct NADPH oxidase-dependent ROS production, and protects against sepsis.
NCBI Summary:	The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q99497
NCBI GenInfo Identifier:	56404943
NCBI Gene ID:	11315
NCBI Accession:	Q99497.2
UniProt Secondary Accession:	Q99497,O14805, Q6DR95, Q7LFU2, B2R4Z1,
UniProt Related Accession:	Q99497
Molecular Weight:	19,891 Da
NCBI Full Name:	Protein/nucleic acid deglycase DJ-1
NCBI Synonym Full Names:	Parkinsonism associated deglycase
NCBI Official Symbol:	PARK7
NCBI Official Synonym Symbols:	DJ1; DJ-1; GATD2; HEL-S-67p
NCBI Protein Information:	protein/nucleic acid deglycase DJ-1
UniProt Protein Name:	Protein/nucleic acid deglycase DJ-1
UniProt Synonym Protein Names:	Maillard deglycase
Protein Family:	DJ-1 protein
UniProt Gene Name:	PARK7

	Western blot analysis of extracts of various cell lines, using PARK7 antibody (CAB0987) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Western blot analysis of extracts from normal (control) and PARK7 knockout (KO) 293T cells, using PARK7 antibody (CAB0987) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
	Immunofluorescence analysis of HeLa cells using PARK7 antibody (CAB0987). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-PARK7 Antibody (CAB18580)	Human DJ-1/PARK 7 ELISA Kit
Anti-PARK7 Antibody (CAB19097)	DJ-1 Colorimetric Cell-Based ELISA Kit
PARK7 Antibody (PACO00708)
PARK7 Antibody (PACO01299)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)