[KO Validated] Prion Protein Rabbit Polyclonal Antibody (CAB18058)
The PRNP Polyclonal Antibody (CAB18058) is a vital tool for researchers studying the prion protein (PRNP) in various biological processes. This antibody, generated in rabbits, exhibits high reactivity towards human samples and has been validated for use in Western blot applications. By specifically binding to the PRNP protein, this antibody enables precise detection and analysis of PRNP in different cell types, making it an essential asset for studies in neuroscience, neurodegenerative diseases, and protein misfolding disorders.PRNP, also known as the cellular prion protein, plays a crucial role in several physiological functions, including neuronal survival, synaptic plasticity, and cellular signaling.
Dysregulation of PRNP has been implicated in diseases such as Creutzfeldt-Jakob disease and Alzheimer's disease, highlighting the importance of investigating its function and regulation. By utilizing the PRNP Polyclonal Antibody, researchers can gain valuable insights into the role of PRNP in neurobiology and potentially identify novel therapeutic targets for treating neurodegenerative disorders.
Product Name:
[KO Validated] Prion Protein Rabbit Polyclonal Antibody
SKU:
CAB18058
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 23-230 of human Prion Protein (NP_001073592.1).
The protein encoded by this gene is a membrane glycosylphosphatidylinositol-anchored glycoprotein that tends to aggregate into rod-like structures. The encoded protein contains a highly unstable region of five tandem octapeptide repeats. This gene is found on chromosome 20, approximately 20 kbp upstream of a gene which encodes a biochemically and structurally similar protein to the one encoded by this gene. Mutations in the repeat region as well as elsewhere in this gene have been associated with Creutzfeldt-Jakob disease, fatal familial insomnia, Gerstmann-Straussler disease, Huntington disease-like 1, and kuru. An overlapping open reading frame has been found for this gene that encodes a smaller, structurally unrelated protein, AltPrp. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
5621
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and Prion Protein knockout (KO) HeLa cells, using [KO Validated] Prion Protein Rabbit pAb (CAB18058) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
