The PRKAR2A Polyclonal Antibody (CAB1531) is designed for research involving PRKAR2A, a protein that plays a critical role in cellular signaling and regulation. The antibody, raised in rabbits, is highly reactive with human samples and is validated for use in Western blot applications. It specifically binds to the PRKAR2A protein, enabling accurate detection and analysis in various cell types, making it ideal for studies in molecular biology and signal transduction research.PRKAR2A, also known as cAMP-dependent protein kinase regulatory subunit, is a key component of the protein kinase A (PKA) signaling pathway, which regulates a wide range of cellular processes including metabolism, gene expression, and cell growth.
Dysregulation of this pathway is implicated in various diseases such as cancer, cardiovascular disorders, and neurological conditions. Investigating the function of PRKAR2A can provide valuable insights into the mechanisms underlying these diseases and potentially lead to the development of targeted therapies.
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER).
Purification Method:
Affinity purification
Gene ID:
5576
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts from normal (control) and PKA RIIα (PRKAR2A)/PKR2 knockout (KO) HeLa cells, using PKA RIIα (PRKAR2A)/PKR2 antibody (CAB1531) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
