The PRKAB1 Polyclonal Antibody (CAB12491) is a valuable tool for researchers studying the protein PRKAB1, which plays a crucial role in regulating cellular energy metabolism. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it an ideal choice for Western blot applications.PRKAB1, also known as AMP-activated protein kinase subunit beta-1, is involved in the activation of AMPK, a master regulator of cellular energy homeostasis. Dysregulation of PRKAB1 has been implicated in various metabolic disorders, including diabetes and obesity.
By targeting PRKAB1 with this antibody, researchers can gain insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets.With its ability to detect PRKAB1 in a variety of cell types, this antibody is a valuable tool for studies in metabolic research, with the potential to uncover new pathways and drug targets for the treatment of metabolic diseases. The validation of this antibody for Western blotting ensures reliable and reproducible results, making it a trusted choice for researchers in the fields of biochemistry and molecular biology.
Product Name:
[KO Validated] AMPKβ1 Rabbit Polyclonal Antibody
SKU:
CAB12491
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-80 of human AMPKβ1 (NP_006244.2).
The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.
Purification Method:
Affinity purification
Gene ID:
5564
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using AMPKβ1 antibody (CAB12491) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
