The KO Validated POR Polyclonal Antibody (CAB8142) is a powerful tool for researchers studying POR, a key enzyme involved in the metabolism of drugs and steroids. This polyclonal antibody is produced in rabbits and has been rigorously validated for use in various applications, including Western blotting.POR, also known as cytochrome P450 oxidoreductase, plays a crucial role in the cytochrome P450 system, which is responsible for metabolizing a wide range of compounds in the body. By targeting POR with this antibody, researchers can accurately detect and analyze POR levels in different tissues and cell types, providing valuable insights into drug metabolism and bioactivation pathways.
The significance of POR in drug metabolism and steroid biosynthesis makes it a valuable target for research in pharmacology, toxicology, and endocrinology. The KO Validated POR Polyclonal Antibody offers researchers a reliable tool for studying POR function and its implications in various health conditions, paving the way for the development of novel therapeutic strategies and personalized medicine approaches.
Product Name:
[KO Validated] CYPOR Rabbit Polyclonal Antibody
SKU:
CAB8142
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human CYPOR (NP_000932.3).
This gene encodes an endoplasmic reticulum membrane oxidoreductase that is essential for multiple metabolic processes, including reactions catalyzed by cytochrome P450 proteins for metabolism of steroid hormones, drugs and xenobiotics. The encoded protein has a flavin adenine dinucleotide (FAD)-binding domain and a flavodoxin-like domain which bind two cofactors, FAD and FMN, that allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene cause a complex set of disorders, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome, that resemble those caused by defects in steroid metabolizing enzymes such as aromatase, 21-hydroxylase, and 17 alpha-hydroxylase.
Purification Method:
Affinity purification
Gene ID:
5447
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KO Validated] CYPOR Rabbit pAb (CAB8142) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
