The KO Validated POLE3 Polyclonal Antibody (CAB6469) is a valuable tool for researchers studying the POLE3 protein, known for its involvement in DNA replication and repair processes. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.The POLE3 protein, a subunit of the DNA polymerase epsilon complex, is essential for maintaining genome stability and integrity. Its function in DNA synthesis and repair makes it a key player in cellular processes such as replication and recombination.
By targeting the POLE3 protein, researchers can gain insight into its role in DNA maintenance and its potential implications in diseases like cancer and genetic disorders.With its specificity and reliability, the KO Validated POLE3 Polyclonal Antibody (CAB6469) offers a powerful tool for investigating the functions and mechanisms of the POLE3 protein in various cellular contexts. Its versatility in detecting POLE3 expression in different cell types makes it an invaluable asset for studies in genetics, oncology, and molecular biology.
Product Name:
[KO Validated] POLE3 Rabbit Polyclonal Antibody
SKU:
CAB6469
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human POLE3 (NP_059139.3).
HepG2,Mouse testis,Mouse bone marrow,Mouse spleen,293T
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
17kDa
Observed MW:
20-25kDa
POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.
Purification Method:
Affinity purification
Gene ID:
54107
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KO Validated] POLE3 Rabbit pAb (CAB6469) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
