The PMS2 Polyclonal Antibody (CAB13680) is a valuable tool for researchers studying the PMS2 protein, an essential component of the DNA mismatch repair system. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the PMS2 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for studies in genetics, cancer research, and other fields where DNA repair mechanisms play a crucial role.PMS2 is a key player in maintaining genomic stability by correcting errors that occur during DNA replication.
Mutations in the PMS2 gene have been linked to Lynch syndrome, a hereditary cancer syndrome characterized by an increased risk of colorectal and other cancers. Research into the function and regulation of PMS2 is essential for understanding the molecular mechanisms underlying cancer development and progression, as well as for identifying potential targets for therapeutic intervention. By using the PMS2 Polyclonal Antibody, researchers can gain valuable insights into the role of PMS2 in DNA repair processes and its implications for human health.
Product Name:
PMS2 Rabbit Polyclonal Antibody
SKU:
CAB13680
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 390-670 of human PMS2 (NP_000526.2).
The protein encoded by this gene is a key component of the mismatch repair system that functions to correct DNA mismatches and small insertions and deletions that can occur during DNA replication and homologous recombination. This protein forms heterodimers with the gene product of the mutL homolog 1 (MLH1) gene to form the MutL-alpha heterodimer. The MutL-alpha heterodimer possesses an endonucleolytic activity that is activated following recognition of mismatches and insertion/deletion loops by the MutS-alpha and MutS-beta heterodimers, and is necessary for removal of the mismatched DNA. There is a DQHA(X)2E(X)4E motif found at the C-terminus of the protein encoded by this gene that forms part of the active site of the nuclease. Mutations in this gene have been associated with hereditary nonpolyposis colorectal cancer (HNPCC; also known as Lynch syndrome) and Turcot syndrome.
Purification Method:
Affinity purification
Gene ID:
5395
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KO Validated] PMS2 Rabbit pAb (CAB13680) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
