The PIP5K1A Polyclonal Antibody (CAB18099) is developed for research related to PIP5K1A, a key enzyme involved in cellular processes such as membrane trafficking and cytoskeletal dynamics. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the PIP5K1A protein, it enables the detection and analysis of PIP5K1A in various cell types, making it well-suited for studies in cell biology and signal transduction pathways.
PIP5K1A, also known as phosphatidylinositol-4-phosphate 5-kinase type I alpha, plays a crucial role in the regulation of phosphoinositide signaling, which is essential for cell proliferation, migration, and survival. Understanding the function of PIP5K1A is important for elucidating its role in diseases such as cancer, neurodegenerative disorders, and insulin resistance. Targeting PIP5K1A could have therapeutic implications in the development of treatments for these conditions.
Product Name:
[KO Validated] PIP5K1A Rabbit Polyclonal Antibody
SKU:
CAB18099
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 231-500 of human PIP5K1A (NP_001129109.1).
Enables 1-phosphatidylinositol-4-phosphate 5-kinase activity and kinase binding activity. Involved in several processes, including activation of GTPase activity; focal adhesion assembly; and ruffle assembly. Located in several cellular components, including lamellipodium; nuclear speck; and ruffle membrane. Colocalizes with mRNA cleavage and polyadenylation specificity factor complex.
Purification Method:
Affinity purification
Gene ID:
8394
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and PIP5K1A knockout (KO) 293T cells, using [KO Validated] PIP5K1A Rabbit pAb (CAB18099) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
