The KO-Validated PIP5K1A Polyclonal Antibody (CAB18025) is a vital tool for researchers studying PIP5K1A, a key enzyme involved in regulating cellular signaling pathways and lipid metabolism. This antibody, produced in rabbits, exhibits strong reactivity with human samples and has been rigorously validated for use in Western blot assays.PIP5K1A, also known as phosphatidylinositol-4-phosphate 5-kinase type 1 alpha, is essential for generating phosphatidylinositol 4,5-bisphosphate (PIP2), a critical lipid messenger involved in various cellular functions like cell growth, motility, and vesicle trafficking.
Dysregulation of PIP5K1A has been linked to diseases such as cancer, diabetes, and neurological disorders, highlighting its importance in cellular homeostasis.By targeting the PIP5K1A protein, this antibody enables researchers to detect and analyze PIP5K1A expression in different cell types, providing valuable insights into its role in physiological and pathological processes. This makes the CAB18025 antibody a valuable tool for advancing research in areas such as cell biology, metabolism, and disease mechanisms involving PIP5K1A.
Product Name:
[KO Validated] PIP5K1A Rabbit Polyclonal Antibody
SKU:
CAB18025
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 231-500 of human PIP5K1A (NP_001129109.1).
Enables 1-phosphatidylinositol-4-phosphate 5-kinase activity and kinase binding activity. Involved in several processes, including activation of GTPase activity; focal adhesion assembly; and ruffle assembly. Located in several cellular components, including lamellipodium; nuclear speck; and ruffle membrane. Colocalizes with mRNA cleavage and polyadenylation specificity factor complex.
Purification Method:
Affinity purification
Gene ID:
8394
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and PIP5K1A knockout (KO) 293T cells, using [KO Validated] PIP5K1A Rabbit pAb (CAB18025) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
