null

[KO Validated] PIP5K1A Rabbit Polyclonal Antibody (CAB18025)

SKU:
CAB18025
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
$167 - $503
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KO Validated] PIP5K1A Rabbit Polyclonal Antibody
SKU:CAB18025
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 231-500 of human PIP5K1A (NP_001129109.1).
Sequence:MNNL LPRS VKMH IKYD LKGS TYKR RASQ KERE KPLP TFKD LDFL QDIP DGLF LDAD MYNA LCKT LQRD CLVL QSFK IMDY SLLM SIHN IDHA QREP LSSE TQYS VDTR RPAP QKAL YSTA MESI QGEA RRGG TMET DDHM GGIP ARNS KGER LLLY IGII DILQ SYRF VKKL EHSW KALV HDGD TVSV HRPG FYAE RFQR FMCN TVFK KIPC VHLG RPDV LPQT PPLE EISE GSPI PDPS FSPL VGET LQML TTST TLEK LEVA ESEF TH
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200
Synonyms:PIP5K1A; 1A
Positive Sample:293T
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Cell projection, Cytoplasm, Nucleus speckle, ruffle.
Calculated MW:63kDa
Observed MW:63kDa

Enables 1-phosphatidylinositol-4-phosphate 5-kinase activity and kinase binding activity. Involved in several processes, including activation of GTPase activity; focal adhesion assembly; and ruffle assembly. Located in several cellular components, including lamellipodium; nuclear speck; and ruffle membrane. Colocalizes with mRNA cleavage and polyadenylation specificity factor complex.

Purification Method:Affinity purification
Gene ID:8394
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from wild type (WT) and PIP5K1A knockout (KO) 293T cells, using [KO Validated] PIP5K1A Rabbit pAb (CAB18025) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

Related Products