The PHKG2 Polyclonal Antibody (CAB19934) is a valuable tool for researchers studying protein kinase G, a key enzyme involved in cellular signaling pathways. This rabbit-raised antibody is highly specific and reactive with human samples, making it ideal for Western blot applications. By binding to the PHKG2 protein, this antibody enables accurate detection and analysis in various cell types, providing valuable insights into the role of protein kinase G in processes such as cell growth, metabolism, and gene expression.Protein kinase G, encoded by the PHKG2 gene, is known to play a crucial role in regulating various cellular functions, making it a potential target for therapeutic interventions in diseases such as cancer, diabetes, and cardiovascular disorders.
Understanding the activity and regulation of PHKG2 is essential for developing targeted therapies that can modulate cellular signaling pathways and improve treatment outcomes in these conditions. The validation of this antibody for use in research provides researchers with a reliable tool for studying the role of PHKG2 in health and disease.
Product Name:
[KO Validated] PHKG2 Rabbit Polyclonal Antibody
SKU:
CAB19934
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 237-406 of human PHKG2 (NP_000285.1).
Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, and the hepatic isoform is encoded by this gene. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9C, also known as autosomal liver glycogenosis. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.
Purification Method:
Affinity purification
Gene ID:
5261
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and PHKG2 knockout (KO) 293T cells, using [KO Validated] PHKG2 Rabbit pAb (CAB19934) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
