The PGM1 Polyclonal Antibody (CAB19904) is a valuable tool for researchers studying PGM1, an enzyme involved in carbohydrate metabolism. This antibody, produced in rabbits, shows high specificity and reactivity with human samples, making it ideal for use in Western blot applications. By binding to the PGM1 protein, this antibody enables accurate detection and analysis in a variety of cell types, providing insights into the role of PGM1 in metabolic pathways and potential implications for diseases such as metabolic disorders and cancer.PGM1 is a key enzyme in the glycolytic pathway, playing a crucial role in the conversion of glucose-1-phosphate to glucose-6-phosphate.
Dysregulation of PGM1 activity has been linked to metabolic disorders such as glycogen storage disease and has also been implicated in the progression of cancer. Research into the function and regulation of PGM1 is important for understanding these diseases and developing targeted therapies. The PGM1 Polyclonal Antibody (CAB19904) offers a reliable tool for investigating PGM1 expression and activity in various experimental settings.
Antibody Name:
Anti-PGM1 Antibody [KO Validated]
Antibody SKU:
CAB19904
Antibody Size:
20uL, 50uL, 100uL
Application:
WB IF
Reactivity:
Human, Mouse, Rat
Host Species:
Rabbit
Immunogen:
Recombinant protein of human PGM1.
Application:
WB IF
Recommended Dilution:
WB 1:500 - 1:2000 IF 1:10 - 1:100
Reactivity:
Human, Mouse, Rat
Positive Samples:
293T
Immunogen:
Recombinant protein of human PGM1.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:
IgG
Sequence:
Email for sequence
Gene ID:
5236
Uniprot:
P36871
Cellular Location:
Cytoplasm
Calculated MW:
40kDa/61kDa/63kDa
Observed MW:
61KDa
Western blot analysis of extracts from normal (control) and PGM1 knockout (KO) 293T cells, using PGM1 antibody (CAB19904) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Synonyms:
PGM1, CDG1T, GSD14
Background:
The protein encoded by this gene is an isozyme of phosphoglucomutase (PGM) and belongs to the phosphohexose mutase family. There are several PGM isozymes, which are encoded by different genes and catalyze the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, this PGM isozyme is predominant, representing about 90% of total PGM activity. In red cells, PGM2 is a major isozyme. This gene is highly polymorphic. Mutations in this gene cause glycogen storage disease type 14. Alternativley spliced transcript variants encoding different isoforms have been identified in this gene.
UniProt Protein Function:
This enzyme participates in both the breakdown and synthesis of glucose.
NCBI Summary:
The protein encoded by this gene is an isozyme of phosphoglucomutase (PGM) and belongs to the phosphohexose mutase family. There are several PGM isozymes, which are encoded by different genes and catalyze the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, this PGM isozyme is predominant, representing about 90% of total PGM activity. In red cells, PGM2 is a major isozyme. This gene is highly polymorphic. Mutations in this gene cause glycogen storage disease type 14. Alternativley spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Mar 2010]
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