The PFKP Polyclonal Antibody (CAB7916) is a valuable tool for researchers studying PFKP, a key enzyme in the glycolytic pathway that plays a critical role in energy metabolism. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By targeting the PFKP protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for studies in metabolism, cancer research, and metabolic diseases.
PFKP is essential for the conversion of fructose-6-phosphate to fructose-1,6-bisphosphate in glycolysis, a process crucial for generating ATP and supporting cell growth and proliferation. Dysregulation of PFKP has been linked to various diseases, including cancer and metabolic disorders. Investigating the role of PFKP using this antibody can provide valuable insights into the mechanisms underlying these conditions and help identify potential therapeutic targets for intervention.
Product Name:
[KO Validated] PFKP Rabbit Polyclonal Antibody
SKU:
CAB7916
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 336-540 of human PFKP (NP_002618.1).
This gene encodes a member of the phosphofructokinase A protein family. The encoded enzyme is the platelet-specific isoform of phosphofructokinase and plays a key role in glycolysis regulation. This gene may play a role in metabolic reprogramming in some cancers, including clear cell renal cell carcinomas, and cancer of the bladder, breast, and lung. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
5214
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using PFKP Rabbit pAb (CAB7916) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
