The PDIA6 Polyclonal Antibody (CAB18092) is a valuable tool for researchers studying PDIA6, a protein involved in protein folding and quality control in the endoplasmic reticulum. This rabbit polyclonal antibody is highly specific and reactive with human samples, making it ideal for use in Western blot applications.PDIA6, also known as protein disulfide isomerase A6, plays a crucial role in maintaining protein homeostasis by catalyzing the formation, breakage, and rearrangement of disulfide bonds within proteins. This function is essential for proper protein folding and preventing protein misfolding, which can lead to diseases such as neurodegenerative disorders and cancer.
By using the PDIA6 Polyclonal Antibody, researchers can study the expression levels and localization of PDIA6 in various cell types and tissues. This information can provide valuable insights into the role of PDIA6 in disease pathogenesis and help guide the development of novel therapeutic strategies targeting protein folding and quality control mechanisms.
Product Name:
[KO Validated] PDIA6 Rabbit Polyclonal Antibody
SKU:
CAB18092
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-300 of human PDIA6 (NP_005733.1).
This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, two catalytically active thioredoxin (TRX) domains, a TRX-like domain, and a C-terminal ER-retention sequence. This protein inhibits the aggregation of misfolded proteins and exhibits both isomerase and chaperone activity. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
10130
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and PDIA6 knockout (KO) 293T cells, using [KO Validated] PDIA6 Rabbit pAb (CAB18092) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
