The PARP Rabbit Monoclonal Antibody (CAB19596) is a powerful tool for researchers studying Poly (ADP-ribose) polymerase (PARP), a critical enzyme involved in various cellular processes such as DNA repair, apoptosis, and genomic stability. This antibody, derived from rabbits, is highly specific and has been validated for use in assays including Western blotting, immunoprecipitation, and immunofluorescence microscopy.PARP plays a key role in DNA damage repair mechanisms, making it an important target for cancer therapeutics and research. The CAB19596 antibody binds specifically to PARP protein, allowing for precise detection and analysis in a variety of cell types and experimental settings.
Its high reactivity with human samples makes it an ideal choice for investigations in oncology, genomics, and drug development.By using the PARP Rabbit Monoclonal Antibody (CAB19596), researchers can gain valuable insights into the function and regulation of PARP in normal and disease states, paving the way for novel therapeutic interventions and personalized medicine approaches. Its versatility and reliability make it a valuable asset for studies that aim to unravel the complexities of PARP biology and its implications in human health.
Product Name:
[KO Validated] PARP1 Rabbit Monoclonal Antibody
SKU:
CAB19596
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 700-800 of human PARP1 (P09874).
Jurkat treated by Staurosporine,Mouse testis,Rat testis
Conjugate:
Unconjugated
Cellular Localization:
Nucleus, nucleolus.
Calculated MW:
113kDa
Observed MW:
89kDa/113kDa
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
Purification Method:
Affinity purification
Gene ID:
142
Clone Number:
ARC0075
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using PARP1 Rabbit mAb (CAB19596) at 1:1000 dilution.Jurkat cells were treated by Staurosporine(1uM) at room temperature for 3 hours .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
