The PACSIN2 Polyclonal Antibody (CAB19941) is a valuable tool for researchers studying PACSIN2, a protein involved in membrane trafficking and cytoskeletal dynamics. This antibody, generated in rabbits, demonstrates high specificity and sensitivity for detecting PACSIN2 in various cell types, making it ideal for use in Western blot applications.PACSIN2, also known as protein kinase C and casein kinase substrate in neurons 2, plays a vital role in endocytosis and vesicle trafficking processes within cells. Its involvement in regulating membrane dynamics and cellular morphology highlights its importance in cell biology research.
By targeting PACSIN2 with this antibody, researchers can investigate its functions and interactions in different cellular contexts, providing insights into its potential roles in various physiological and pathological conditions.The validation of this antibody for use in Western blotting ensures reliable and reproducible results, allowing for accurate detection and analysis of PACSIN2 protein expression. With its versatility and specificity, the PACSIN2 Polyclonal Antibody is a valuable asset for studies exploring membrane dynamics, vesicle trafficking, and cellular signaling pathways.
Product Name:
[KO Validated] PACSIN2 Rabbit Polyclonal Antibody
SKU:
CAB19941
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 240-410 of human PACSIN2 (NP_009160.2).
This gene is a member of the protein kinase C and casein kinase substrate in neurons family. The encoded protein is involved in linking the actin cytoskeleton with vesicle formation by regulating tubulin polymerization. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
11252
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and PACSIN2 knockout (KO) HeLa cells, using [KO Validated] PACSIN2 Rabbit pAb (CAB19941) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
