The KO Validated p53 Rabbit Monoclonal Antibody (CAB19585) is a cutting-edge tool for researchers studying the tumor suppressor protein p53. This antibody, produced in rabbits, has been rigorously tested for knockout validation, ensuring its specificity and accuracy in detecting p53 in knockout cell lines.With its high reactivity in human samples, the p53 antibody is an essential tool for Western blot applications, allowing for precise analysis of p53 expression levels in various experimental settings. Its ability to bind specifically to the p53 protein makes it a reliable option for researchers investigating the role of p53 in cancer development, progression, and treatment.
The tumor suppressor protein p53 is a key player in regulating cell growth and preventing tumor formation. Dysregulation of p53 is commonly observed in various types of cancer, highlighting the importance of studying its functions and mechanisms of action. The KO Validated p53 Rabbit Monoclonal Antibody provides researchers with a valuable tool for elucidating the complex role of p53 in cancer biology and potentially identifying novel therapeutic targets for cancer treatment.
Product Name:
[KO Validated] p53 Rabbit Monoclonal Antibody
SKU:
CAB19585
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-393 of human p53 (NP_000537.3).
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
Purification Method:
Affinity purification
Gene ID:
7157
Clone Number:
ARC56085
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells using [KO Validated] p53 Rabbit mAb (CAB19585) at 1:1000 dilution .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020)Exposure time: 1s.
