The NUDT1 Polyclonal Antibody (CAB13330) is a valuable tool for researchers studying NUDT1, a protein involved in DNA repair and oxidative stress response. This antibody, generated in rabbits, is highly specific for human samples and has been rigorously validated for Western blot applications. By targeting the NUDT1 protein, this antibody enables precise detection and analysis in various cell types, making it ideal for investigations in DNA damage, cancer biology, and oxidative stress pathways.NUDT1, also known as nudix hydrolase 1, plays a crucial role in maintaining genomic integrity by hydrolyzing potentially mutagenic nucleotide metabolites. Its involvement in DNA repair processes and cellular defense against oxidative damage highlights its significance in cancer development and response to chemotherapy.
Research on NUDT1 can provide insights into mechanisms of DNA repair, cellular survival under genotoxic stress, and potential therapeutic targets for cancer treatment.Overall, the NUDT1 Polyclonal Antibody (CAB13330) offers researchers a reliable tool for investigating the functions and regulatory mechanisms of NUDT1 in various biological contexts. Its specificity and sensitivity make it a valuable asset for studies aiming to unravel the complex roles of NUDT1 in DNA repair pathways and cancer biology.
Product Name:
[KO Validated] MTH1 Rabbit Polyclonal Antibody
SKU:
CAB13330
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-179 of human MTH1 (NP_945192.1).
WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
MTH1; H1
Positive Sample:
SW480,K562,22RV1,THP-1,HepG2,SKOV3,293T
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Mitochondrion matrix, Nucleus.
Calculated MW:
18kDa
Observed MW:
18kDa
Misincorporation of oxidized nucleoside triphosphates into DNA/RNA during replication and transcription can cause mutations that may result in carcinogenesis or neurodegeneration. The protein encoded by this gene is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy rATP, to monophosphates, thereby preventing misincorporation. The encoded protein is localized mainly in the cytoplasm, with some in the mitochondria, suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. Several alternatively spliced transcript variants, some of which encode distinct isoforms, have been identified. Additional variants have been observed, but their full-length natures have not been determined. A rare single-nucleotide polymorphism that results in the production of an additional, longer isoform (p26) has been described.
Purification Method:
Affinity purification
Gene ID:
4521
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KO Validated] MTH1 Rabbit pAb (CAB13330) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
