The KO Validated Notch1 Polyclonal Antibody (CAB7636) is a valuable tool for researchers studying Notch1, a critical cell signaling pathway implicated in various cellular processes, including development, differentiation, and disease progression. This antibody, produced in rabbits, demonstrates high reactivity with Notch1 in human samples and is specifically validated for use in Western blot applications.Notch1 is a transmembrane receptor that plays a key role in cell fate determination, stem cell maintenance, and tissue homeostasis. Dysregulation of the Notch1 pathway has been linked to numerous diseases, including cancer, developmental disorders, and cardiovascular conditions.
The ability to detect and analyze Notch1 protein levels in various cell types using this antibody makes it an essential tool for research in the fields of developmental biology, oncology, and regenerative medicine.By understanding the mechanisms by which Notch1 signaling influences cellular behavior, researchers can gain valuable insights into disease pathogenesis and identify potential therapeutic targets for intervention. The KO Validated Notch1 Polyclonal Antibody (CAB7636) provides a reliable and specific means of investigating the role of Notch1 in health and disease, paving the way for advancements in personalized medicine and targeted therapeutics.
Product Name:
[KO Validated] Notch1 Rabbit Polyclonal Antibody
SKU:
CAB7636
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 2456-2555 of human Notch1 (NP_060087.3).
Cell membrane, Nucleus, Single-pass type I membrane protein.
Calculated MW:
273kDa
Observed MW:
300kDa
This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor plays a role in the development of numerous cell and tissue types. Mutations in this gene are associated with aortic valve disease, Adams-Oliver syndrome, T-cell acute lymphoblastic leukemia, chronic lymphocytic leukemia, and head and neck squamous cell carcinoma.
Purification Method:
Affinity purification
Gene ID:
4851
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat brain , using Notch1 Rabbit pAb (CAB7636) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
