The NF2 Polyclonal Antibody (CAB0739) is a valuable tool for researchers studying NF2, a tumor suppressor gene associated with neurofibromatosis type 2. This antibody, produced in rabbits, exhibits high specificity and sensitivity for human samples, making it an ideal choice for Western blot applications. By binding to the NF2 protein, this antibody allows for precise detection and analysis in a variety of cell types, enabling further exploration of NF2 function in both normal and disease states.NF2, also known as Merlin, plays a crucial role in cell growth regulation and tumor suppression, particularly in the nervous system.
Dysregulation of NF2 has been linked to the development of various tumors, making it a key focus in cancer research. Understanding the mechanisms by which NF2 functions can provide insights into potential therapeutic targets for treating NF2-related disorders and cancers. The NF2 Polyclonal Antibody is a valuable resource for scientists seeking to unravel the complexities of NF2 biology and its implications for human health.
Product Name:
[KO Validated] NF2 Rabbit Polyclonal Antibody
SKU:
CAB0739
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 477-576 of human NF2 (NP_000259.1).
This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that link cytoskeletal components with proteins in the cell membrane. The encoded protein is involved in regulation of contact-dependent inhibition of cell proliferation and functions in cell-cell adhesion and transmembrane signaling. The encoded protein has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics, and proteins involved in regulating ion transport. Disruption of this protein's function has been implicated in tumorigenesis and metastasis. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities.
Purification Method:
Affinity purification
Gene ID:
4771
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and NF2 knockdown (KD) HeLa cells using [KD Validated] NF2 Rabbit pAb (CAB0739) at 1:1000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020)Exposure time: 180 s.
