The NDUFB5 Polyclonal Antibody (CAB19953) is a valuable tool for researchers studying NADH dehydrogenase (ubiquinone) 1 beta subcomplex subunit 5 (NDUFB5), a critical component of mitochondrial complex I involved in electron transport and ATP production. This antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to NDUFB5 protein, this antibody enables accurate detection and analysis in various cell types, making it ideal for investigations in mitochondrial biology and energy metabolism research.
NDUFB5 plays a crucial role in the functioning of complex I, the first and largest enzyme complex of the mitochondrial electron transport chain. Dysregulation of complex I activity, including NDUFB5, has been linked to a variety of human diseases, such as neurodegenerative disorders and metabolic syndromes. Therefore, exploring the role of NDUFB5 in mitochondrial function is essential for gaining insights into these pathologies and developing potential therapeutic strategies targeting mitochondrial dysfunction.
Product Name:
[KO Validated] NDUFB5 Rabbit Polyclonal Antibody
SKU:
CAB19953
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 100-189 of human NDUFB5 (NP_002483.1).
The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Three transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
4711
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and NDUFB5 knockout (KO) HeLa cells, using [KO Validated] NDUFB5 Rabbit pAb (CAB19953) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
