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[KO Validated] NDUFB4 Rabbit Polyclonal Antibody (CAB13820)

SKU:
CAB13820
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • KO Validated Antibodies 1 Anti-NDUFB4 Antibody CAB13820KO Validated
  • KO Validated Antibodies 1 Anti-NDUFB4 Antibody CAB13820KO Validated
  • KO Validated Antibodies 1 Anti-NDUFB4 Antibody CAB13820KO Validated
  • KO Validated Antibodies 1 Anti-NDUFB4 Antibody CAB13820KO Validated
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KO Validated] NDUFB4 Rabbit Polyclonal Antibody
SKU:CAB13820
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human NDUFB4 (NP_001161803.1).
Sequence:MSFP KYKP SSLR TLPE TLDP AEYN ISPE TRRA QAER LAIR AQLK REYL LQYN DPNR RGLI ENPA LLRW AYAR TINV YPNF RPTP KNSL MG
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:100
Synonyms:B15; CI-B15; B4
Positive Sample:U-87MG,HeLa,293T,Mouse heart,Mouse kidney,Mouse liver
Conjugate:Unconjugated
Cellular Localization:Matrix side, Mitochondrion inner membrane, Single-pass membrane protein.
Calculated MW:15kDa
Observed MW:15kDa

This gene encodes a non-catalytic subunit of the multisubunit NADH:ubiquinone oxidoreductase, the first enzyme complex in the mitochondrial electron transport chain (complex I). Mammalian complex I is composed of 45 different subunits and transfers electrons from NADH to ubiquinone.

Purification Method:Affinity purification
Gene ID:4710
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using [KO Validated] NDUFB4 Rabbit pAb (CAB13820) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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