The NDUFB4 Polyclonal Antibody (CAB13820) is a valuable tool for researchers studying NDUFB4, a subunit of the NADH:ubiquinone oxidoreductase (Complex I) enzyme complex involved in cellular respiration and energy production. This antibody, generated in rabbits, has high specificity for human samples and has been validated for use in Western blotting applications.NDUFB4 plays a key role in the electron transport chain and is essential for ATP synthesis in mitochondria. Dysregulation of NDUFB4 has been linked to various diseases, including neurodegenerative disorders and metabolic syndromes.
The ability to detect and quantify NDUFB4 levels in different cell types allows for detailed investigation into its function and potential therapeutic implications.By utilizing the NDUFB4 Polyclonal Antibody, researchers can gain insight into the molecular mechanisms underlying mitochondrial function and energy metabolism. This antibody is an essential tool for studies in biochemistry, cell biology, and disease research related to mitochondrial dysfunction and energy deficiency.
Product Name:
[KO Validated] NDUFB4 Rabbit Polyclonal Antibody
SKU:
CAB13820
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human NDUFB4 (NP_001161803.1).
This gene encodes a non-catalytic subunit of the multisubunit NADH:ubiquinone oxidoreductase, the first enzyme complex in the mitochondrial electron transport chain (complex I). Mammalian complex I is composed of 45 different subunits and transfers electrons from NADH to ubiquinone.
Purification Method:
Affinity purification
Gene ID:
4710
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KO Validated] NDUFB4 Rabbit pAb (CAB13820) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
