The NCOA2 Polyclonal Antibody (CAB10280) is designed for research involving NCOA2, a protein known for its role in gene transcription and cell growth regulation. The antibody, raised in rabbits, is highly reactive with human samples and is validated for use in Western blot applications. It binds specifically to the NCOA2 protein, allowing for accurate detection and analysis in various cell types, making it ideal for studies in molecular biology and cancer research.
NCOA2, also known as nuclear receptor coactivator 2, is a key player in the regulation of gene expression and cellular processes. Its involvement in various signaling pathways and its impact on cell proliferation and differentiation make it a valuable target for research into cancer, metabolic disorders, and developmental biology. Understanding the function of NCOA2 is essential for gaining insights into disease mechanisms and potential therapeutic interventions.
Product Name:
[KO Validated] NCOA2 Rabbit Polyclonal Antibody
SKU:
CAB10280
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 550-820 of human NCOA2 (NP_006531.1).
The protein encoded by this gene functions as a transcriptional coactivator for nuclear hormone receptors, including steroid, thyroid, retinoid, and vitamin D receptors. The encoded protein acts as an intermediary factor for the ligand-dependent activity of these nuclear receptors, which regulate their target genes upon binding of cognate response elements. This gene has been found to be involved in translocations that result in fusions with other genes in various cancers, including the lysine acetyltransferase 6A (KAT6A) gene in acute myeloid leukemia, the ETS variant 6 (ETV6) gene in acute lymphoblastic leukemia, and the hes related family bHLH transcription factor with YRPW motif 1 (HEY1) gene in mesenchymal chondrosarcoma. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
10499
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and NCOA2 knockout (KO) 293T cells, using [KO Validated] NCOA2 Rabbit pAb (CAB10280) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
