The MTA2 Rabbit Monoclonal Antibody (CAB4624) is a high-quality antibody designed for research involving MTA2, a key component of the nucleosome remodeling and histone deacetylation (NuRD) complex. This antibody, validated for use in various applications including Western blot and immunohistochemistry, specifically targets the MTA2 protein in human samples.MTA2 is known for its role in chromatin remodeling and gene regulation, making it a valuable target for studies in cancer research and epigenetics. By detecting and analyzing MTA2 expression in different cell types, researchers can gain insights into the mechanisms underlying gene silencing and transcriptional regulation, which are critical for understanding cancer progression and drug resistance.
The MTA2 Rabbit Monoclonal Antibody (CAB4624) offers researchers a reliable tool for investigating the functions of MTA2 in various biological contexts. Its high specificity and sensitivity make it a valuable resource for studies aiming to unravel the complex interplay between chromatin remodeling and gene expression in health and disease.
Product Name:
[KO Validated] MTA2 Rabbit Monoclonal Antibody
SKU:
CAB4624
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 569-668 of human MTA2 (O94776).
Sequence:
GVPF SANG RPLA SGIR SSSQ PAAK RQKL NPAD APNP VVFV ATKD TRAL RKAL THLE MRRA ARRP NLPL KVKP TLIA VRPP VPLP APSH PAST NEPI VLED
Tested Applications:
WBIHC-PIF/ICCChIPELISA
Recommended Dilution:
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 ChIP,5μg antibody for 10μg-15μg of Chromatin
Synonyms:
PID; MTA1L1; A2
Positive Sample:
HeLa,293T
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
75kDa
Observed MW:
75kDa
This gene encodes a protein that has been identified as a component of NuRD, a nucleosome remodeling deacetylase complex identified in the nucleus of human cells. It shows a very broad expression pattern and is strongly expressed in many tissues. It may represent one member of a small gene family that encode different but related proteins involved either directly or indirectly in transcriptional regulation. Their indirect effects on transcriptional regulation may include chromatin remodeling. It is closely related to another member of this family, a protein that has been correlated with the metastatic potential of certain carcinomas. These two proteins are so closely related that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. One of the proteins known to be a target protein for this gene product is p53. Deacetylation of p53 is correlated with a loss of growth inhibition in transformed cells supporting a connection between these gene family members and metastasis.
Purification Method:
Affinity purification
Gene ID:
9219
Clone Number:
ARC1056
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using MTA2 Rabbit mAb (CAB4624) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
