The KO Validated METTL3 Rabbit Monoclonal Antibody (CAB19079) is a powerful tool for researchers studying METTL3, a key enzyme involved in mRNA methylation and gene regulation. This monoclonal antibody, created using rabbit-derived cells, is highly specific and sensitive, making it an ideal choice for applications such as Western blotting and immunohistochemistry.METTL3 plays a critical role in various cellular processes, including RNA metabolism, protein synthesis, and epigenetic regulation. Dysregulation of METTL3 has been linked to numerous diseases, including cancer, neurological disorders, and metabolic diseases.
By targeting METTL3 with this validated antibody, researchers can gain valuable insights into its function and potential therapeutic applications.Whether investigating the role of METTL3 in cancer progression, neurological development, or metabolic dysfunction, the KO Validated METTL3 Rabbit Monoclonal Antibody (CAB19079) offers researchers a reliable and accurate tool for their studies. Trust in this antibody to deliver precise and reproducible results, advancing our understanding of METTL3 biology and its impact on human health.
Product Name:
[KO Validated] METTL3 Rabbit Monoclonal Antibody
SKU:
CAB19079
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 2-250 of human METTL3 (Q86U44).
WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
M6A; IME4; Spo8; MT-A70; hMETTL3; METTL3
Positive Sample:
HeLa,293F
Conjugate:
Unconjugated
Cellular Localization:
Nucleus speckle.
Calculated MW:
64kDa
Observed MW:
80kDa/70kDa
This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine.
Purification Method:
Affinity purification
Gene ID:
56339
Clone Number:
ARC0487
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using METTL3 Rabbit mAb (CAB19079) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 3min.
