The MDH2 Polyclonal Antibody (CAB19906) is a powerful tool for researchers studying malate dehydrogenase 2 (MDH2), an essential enzyme involved in cellular energy production. This antibody, raised in rabbits, has been validated for use in various applications, including Western blotting and immunohistochemistry.MDH2 plays a key role in the citric acid cycle, where it catalyzes the conversion of malate to oxaloacetate, generating NADH for ATP production. Dysregulation of MDH2 has been linked to various diseases, including cancer and metabolic disorders, making it a promising target for therapeutic interventions.
By specifically binding to MDH2, this antibody enables researchers to detect and analyze the expression levels of the enzyme in different cell types and tissues. This information is crucial for understanding the role of MDH2 in cellular metabolism and its implications for disease progression.Overall, the MDH2 Polyclonal Antibody is a valuable tool for researchers investigating the biology of MDH2 and its potential as a therapeutic target in various diseases. Its high specificity and sensitivity make it a reliable choice for studies in biochemistry, cell biology, and cancer research.
Product Name:
[KO Validated] MDH2 Rabbit Polyclonal Antibody
SKU:
CAB19906
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 59-338 of human MDH2 (NP_005909.2).
Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
4191
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts from normal (control) and MDH2 knockout (KO) 293T cells, using MDH2 antibody (CAB19906) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
