Anti-MCL1 Antibody (CAB18001)[KO Validated]
- SKU:
- CAB18001
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Death
Description
Anti-MCL1 Antibody (CAB18001)[KO Validated]
The MCL1 Polyclonal Antibody (CAB18001) is a valuable tool for researchers studying MCL1, a protein known for its role in regulating cell survival and apoptosis. Raised in rabbits, this antibody is highly specific to human samples and has been validated for use in Western blot applications. By binding to the MCL1 protein, it enables accurate detection and analysis in a variety of cell types, making it ideal for studies in cancer biology and apoptosis research.MCL1 is a key player in the process of programmed cell death, making it a crucial target for research into cancer and other diseases where abnormal cell survival is a factor.
By studying the activity and regulation of MCL1, researchers can gain valuable insights into potential therapeutic strategies for targeting this protein in cancer treatment. The MCL1 Polyclonal Antibody is a reliable tool for investigating the function of MCL1 and its potential as a target for drug development in oncology.
| Antibody Name: | Anti-MCL1 Antibody [KO Validated] |
| Antibody SKU: | CAB18001 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide of human MCL1 |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549 |
| Immunogen: | A synthetic peptide of human MCL1 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 4170 |
| Uniprot: | Q07820 |
| Cellular Location: | Cytoplasm, Membrane, Mitochondrion, Nucleus, Single-pass membrane protein, nucleoplasm |
| Calculated MW: | 28kDa/37kDa |
| Observed MW: | 40kDa |
![Anti-MCL1 Antibody [KO Validated] (CAB18001)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/p/527/A18001_1__02876.jpg) | Western blot analysis of extracts from normal (control) and MCL1 knockout (KO) A-549 cells, using MCL1 antibody (CAB18001) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
| Synonyms: | MCL1, BCL2L3, EAT, MCL1-ES, MCL1L, MCL1S, Mcl-1, TM, bcl2-L-3, mcl1/EAT, MCL1 |
| Background: | This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. |
| UniProt Protein Function: | MCL1: a myeloid cell leukemia protein of the Bcl-2 family of proteins. Two alternatively spliced transcripts encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing. |
| UniProt Protein Details: | Protein type:Inhibitor; Apoptosis; Mitochondrial; Channel, misc.; Membrane protein, integral Chromosomal Location of Human Ortholog: 1q21 Cellular Component: nucleoplasm; mitochondrial outer membrane; mitochondrion; membrane; mitochondrial matrix; cytoplasm; integral to membrane; cytosol; nucleus Molecular Function:protein binding; protein homodimerization activity; protein heterodimerization activity; protein channel activity; BH3 domain binding Biological Process: apoptotic mitochondrial changes; DNA damage response, signal transduction resulting in induction of apoptosis; response to cytokine stimulus; multicellular organismal development; cellular homeostasis; cell fate determination |
| NCBI Summary: | This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010] |
| UniProt Code: | Q07820 |
| NCBI GenInfo Identifier: | 83304396 |
| NCBI Gene ID: | 4170 |
| NCBI Accession: | Q07820.3 |
| UniProt Secondary Accession: | Q07820,Q9HD91, Q9NRQ3, Q9NRQ4, Q9UHR7, Q9UHR8, Q9UHR9 Q9UNJ1, B2R6B2, D3DV03, D3DV04, |
| UniProt Related Accession: | Q07820 |
| Molecular Weight: | 350 |
| NCBI Full Name: | Induced myeloid leukemia cell differentiation protein Mcl-1 |
| NCBI Synonym Full Names: | myeloid cell leukemia 1 |
| NCBI Official Symbol: | MCL1Â Â |
| NCBI Official Synonym Symbols: | TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EATÂ Â |
| NCBI Protein Information: | induced myeloid leukemia cell differentiation protein Mcl-1; bcl-2-like protein 3; myeloid cell leukemia ES; bcl-2-related protein EAT/mcl1; myeloid cell leukemia sequence 1 (BCL2-related) |
| UniProt Protein Name: | Induced myeloid leukemia cell differentiation protein Mcl-1 |
| UniProt Synonym Protein Names: | Bcl-2-like protein 3; Bcl2-L-3; Bcl-2-related protein EAT/mcl1; mcl1/EAT |
| Protein Family: | Minichromosome loss protein |
| UniProt Gene Name: | MCL1Â Â |
| UniProt Entry Name: | MCL1_HUMAN |
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