The MCAM Polyclonal Antibody (CAB19993) is ideal for researchers studying MCAM, a cell adhesion molecule known for its role in cancer progression and metastasis. This rabbit-derived antibody is highly specific to human samples and has been validated for use in Western blot applications. By binding to the MCAM protein, this antibody enables the detection and analysis of MCAM expression in various cell types, making it a valuable tool for studies in oncology and tumor biology.MCAM, also known as melanoma cell adhesion molecule, is involved in cell-to-cell interactions and is overexpressed in a variety of cancer types, including melanoma, breast cancer, and prostate cancer.
Its association with cancer aggressiveness and metastasis makes it a promising target for therapeutic interventions aimed at inhibiting tumor growth and spread. Researchers interested in investigating the role of MCAM in cancer biology will find this antibody essential for their experiments.
Involved in glomerular filtration and vascular wound healing. Acts upstream of or within angiogenesis. Located in external side of plasma membrane. Biomarker of chronic obstructive pulmonary disease and uveal melanoma.
Purification Method:
Affinity purification
Gene ID:
4162
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and CD146/CD146/MCAM knockout (KO) HeLa cells, using [KO Validated] CD146/MCAM Rabbit pAb (CAB19993) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
