Anti-MAP3K1 Antibody (CAB18041)[KO Validated]
- SKU:
- CAB18041
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
Anti-MAP3K1 Antibody (CAB18041)[KO Validated]
The MAP3K1 Polyclonal Antibody (CAB18041) is a reliable tool for researchers studying the mitogen-activated protein kinase kinase kinase 1 (MAP3K1) protein, a key regulator of cellular signaling pathways. This antibody, generated in rabbits, has been validated for use in various experimental applications, particularly Western blot analysis. It specifically binds to the MAP3K1 protein, enabling precise detection and characterization in different cell types.MAP3K1 is a crucial component of signaling cascades involved in cell growth, differentiation, and survival. Dysregulation of MAP3K1 activity has been linked to various diseases, including cancer, making it a promising target for therapeutic intervention.
By investigating the function and regulation of MAP3K1, researchers can gain insights into its role in disease pathogenesis and potentially develop targeted treatment strategies.Overall, the MAP3K1 Polyclonal Antibody (CAB18041) is a valuable tool for scientists studying signal transduction pathways and the molecular mechanisms underlying disease development. Its high specificity and sensitivity make it an essential reagent for immunology, cancer biology, and drug discovery research.
| Antibody Name: | Anti-MAP3K1 Antibody [KO Validated] |
| Antibody SKU: | CAB18041 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1400 to the C-terminus of human MAP3K1 (NP_005912.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HeLa |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1400 to the C-terminus of human MAP3K1 (NP_005912.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TGAG EFQG QLLG TIAF MAPE VLRG QQYG RSCD VWSV GCAI IEMA CAKP PWNA EKHS NHLA LIFK IASA TTAP SIPS HLSP GLRD VALR CLEL QPQD RPPS RELL KHPV FRTT W |
| Gene ID: | 4214 |
| Uniprot: | Q13233 |
| Cellular Location: | |
| Calculated MW: | 164kDa |
| Observed MW: | 164kDa |
![Anti-MAP3K1 Antibody [KO Validated] (CAB18041)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/f/516/A18041_1__47842.jpg) | Western blot analysis of extracts from normal (control) and MAP3K1 knockout (KO) HeLa cells, using MAP3K1 antibody (CAB18041) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1min. |
| Synonyms: | MAP3K1, MAPKKK1, MEKK, MEKK 1, MEKK1, SRXY6 |
| Background: | The protein encoded by this gene is a serine/threonine kinase and is part of some signal transduction cascades, including the ERK and JNK kinase pathways as well as the NF-kappa-B pathway. The encoded protein is activated by autophosphorylation and requires magnesium as a cofactor in phosphorylating other proteins. This protein has E3 ligase activity conferred by a plant homeodomain (PHD) in its N-terminus and phospho-kinase activity conferred by a kinase domain in its C-terminus. |
| UniProt Protein Function: | MEKK1: a protein kinase of the STE11 family. Phosphorylates and activates MKK4 and -7, which in turn activate JNK1, -2 and -3. Contains 1 RING-type zinc finger. The RING finger domain of MEKK1 exhibits E3 ubiquitin ligase activity toward c-Jun. c-Jun is downregulated in response to osmotic stress via ubiquitination-mediated degradation by the RING finger domain of MEKK1. Overexpressed/overactivated in multiple tumor types. The Mek1/2 inhibitor U0126 blocks export of influenza viral particles and has been suggested as an antiviral treatment. Single mutations at the same point of each gene also associated with cardiofaciocutaneous syndrome, (also linked with Braf and Kras mutations), displaying morphological, cardiac and mental defects. Inhibitors: U0126, CI-1040/PD184352, PD-0325901 (Phase I cancer), ARRY-142886 (Phase 1, cancer). |
| UniProt Protein Details: | Protein type:Ubiquitin ligase; Ligase; EC 2.7.11.25; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Ubiquitin conjugating system; STE group; STE11 family Chromosomal Location of Human Ortholog: 5q11.2 Cellular Component: cytosol Molecular Function:protein binding; zinc ion binding; MAP kinase kinase kinase activity; JUN kinase kinase activity; protein kinase binding; ATP binding; protein kinase activity Biological Process: wound healing; activation of MAPKK activity; apoptosis; regulation of cell migration; protein amino acid phosphorylation; activation of JNK activity; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; apoptotic mitochondrial changes; positive regulation of actin filament polymerization; transforming growth factor beta receptor signaling pathway; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; actin cytoskeleton organization and biogenesis; toll-like receptor 4 signaling pathway Disease: 46,xy Sex Reversal 6 |
| NCBI Summary: | The protein encoded by this gene is a serine/threonine kinase and is part of some signal transduction cascades, including the ERK and JNK kinase pathways as well as the NF-kappa-B pathway. The encoded protein is activated by autophosphorylation and requires magnesium as a cofactor in phosphorylating other proteins. This protein has E3 ligase activity conferred by a plant homeodomain (PHD) in its N-terminus and phospho-kinase activity conferred by a kinase domain in its C-terminus. [provided by RefSeq, Mar 2012] |
| UniProt Code: | Q13233 |
| NCBI GenInfo Identifier: | 218512139 |
| NCBI Gene ID: | 4214 |
| NCBI Accession: | Q13233.4 |
| UniProt Related Accession: | Q13233 |
| Molecular Weight: | 164,470 Da |
| NCBI Full Name: | Mitogen-activated protein kinase kinase kinase 1 |
| NCBI Synonym Full Names: | mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase |
| NCBI Official Symbol: | MAP3K1Â Â |
| NCBI Official Synonym Symbols: | MEKK; MEKK1; SRXY6; MEKK 1; MAPKKK1Â Â |
| NCBI Protein Information: | mitogen-activated protein kinase kinase kinase 1; MEK kinase 1; MAP/ERK kinase kinase 1; MAPK/ERK kinase kinase 1 |
| UniProt Protein Name: | Mitogen-activated protein kinase kinase kinase 1 |
| UniProt Synonym Protein Names: | MAPK/ERK kinase kinase 1; MEK kinase 1; MEKK 1 |
| UniProt Gene Name: | MAP3K1Â Â |
| UniProt Entry Name: | M3K1_HUMAN |
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