The MAP2K1 Polyclonal Antibody (CAB12687) is a valuable tool for research involving MAP2K1, a key protein involved in the MAPK signaling pathway. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blotting applications. By targeting the MAP2K1 protein, this antibody enables researchers to detect and analyze MAP2K1 expression in various cell types, making it ideal for studies in cell signaling, cancer research, and drug development.MAP2K1, also known as MEK1, plays a crucial role in transmitting signals from cell surface receptors to the nucleus, ultimately influencing cellular processes such as proliferation, differentiation, and survival.
Dysregulation of the MAPK signaling pathway, in which MAP2K1 is a key player, has been implicated in various diseases including cancer, developmental disorders, and neurological conditions. Understanding the function of MAP2K1 and its interactions within the MAPK pathway is essential for developing targeted therapies and identifying potential drug targets for these diseases.
Product Name:
[KO Validated] MEK1 Rabbit Polyclonal Antibody
SKU:
CAB12687
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MEK1 (NP_002746.1).
Cytoplasm, Membrane, Nucleus, Peripheral membrane protein, centrosome, cytoskeleton, microtubule organizing center, spindle pole body.
Calculated MW:
43kDa
Observed MW:
45kDa
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
Purification Method:
Affinity purification
Gene ID:
5604
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KO Validated] MEK1 Rabbit pAb (CAB12687) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
