The KO Validated MacroH2A.1 Rabbit Polyclonal Antibody (CAB18091) is a valuable tool for researchers studying MacroH2A.1, a histone variant involved in chromatin structure and gene regulation. This antibody, produced in rabbits, is highly specific and reacts strongly with human samples, making it ideal for use in Western blot applications.MacroH2A.1 is known to play key roles in processes such as X chromosome inactivation, DNA repair, and cellular differentiation. Its expression has been linked to various diseases, including cancer and age-related disorders.
By targeting MacroH2A.1 with this antibody, researchers can investigate its function and potential implications in different biological contexts.With its high specificity and reliability in Western blotting, the KO Validated MacroH2A.1 Rabbit Polyclonal Antibody offers a valuable tool for studying the role of MacroH2A.1 in chromatin dynamics and gene regulation. Its validation in knockout models ensures accurate and reproducible results, making it a trusted reagent for research in molecular biology, epigenetics, and disease mechanisms.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
9555
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and macroH2A.1 knockout (KO) 293T cells, using [KO Validated] macroH2A.1 Rabbit pAb (CAB18091) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
