[KO Validated] DNA Ligase IV Rabbit Polyclonal Antibody (CAB18050)
- SKU:
- CAB18050
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Cycle
Frequently bought together:
Description
Product Name: | [KO Validated] DNA Ligase IV Rabbit Polyclonal Antibody |
SKU: | CAB18050 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 612-911 of human DNA Ligase IV (NP_002303.2). |
Sequence: | SKHL YIGG DDEP QEKK RKAA PKMK KVIG IIEH LKAP NLTN VNKI SNIF EDVE FCVM SGTD SQPK PDLE NRIA EFGG YIVQ NPGP DTYC VIAG SENI RVKN IILS NKHD VVKP AWLL ECFK TKSF VPWQ PRFM IHMC PSTK EHFA REYD CYGD SYFI DTDL NQLK EVFS GIKN SNEQ TPEE MASL IADL EYRY SWDC SPLS MFRR HTVY LDSY AVIN DLST KNEG TRLA IKAL ELRF HGAK VVSC LAEG VSHV IIGE DHSR VADF KAFR RTFK RKFK ILKE SWVT DSID KCEL QEEN QYLI |
Tested Applications: | WB ELISA |
Recommended Dilution: | WB,1:500 - 1:2000 |
Synonyms: | LIG4S; IV |
Positive Sample: | HeLa |
Conjugate: | Unconjugated |
Cellular Localization: | Nucleus. |
Calculated MW: | 104kDa |
Observed MW: | 100kDa |
The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.
Purification Method: | Affinity purification |
Gene ID: | 3981 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |