The KO Validated LC3B Rabbit Monoclonal Antibody (CAB19665) is a cutting-edge tool for researchers studying autophagy, a cellular process essential for maintaining cellular homeostasis and responding to stress. This antibody, generated in rabbits, is highly specific for the LC3B protein, a key marker of autophagosomes, and has been rigorously validated for knockout (KO) studies.LC3B is a critical protein involved in the formation and maturation of autophagosomes, the structures responsible for engulfing and degrading cellular components. Dysregulation of autophagy has been implicated in various diseases, including neurodegenerative disorders, cancer, and metabolic syndromes.
By targeting LC3B with this antibody, researchers can effectively track and quantify autophagic activity in different experimental systems.The KO Validated LC3B Rabbit Monoclonal Antibody is ideal for use in techniques such as immunofluorescence, immunoprecipitation, and flow cytometry, providing valuable insights into the dynamics of autophagy in various cell types and conditions. With its high specificity and reliability, this antibody is a valuable asset for advancing autophagy research and discovering potential therapeutic targets for autophagy-related diseases.
Product Name:
[KO Validated] LC3B Rabbit Monoclonal Antibody
SKU:
CAB19665
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 2-107 of human LC3B (Q9GZQ8).
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
Purification Method:
Affinity purification
Gene ID:
81631
Clone Number:
ARC0144
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using [KD Validated] LC3B Rabbit mAb (CAB19665) at 1:1000 dilution.293T, C6 and NIH/3T3 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
