The KO-validated Lamin A/C Rabbit Monoclonal Antibody (CAB19524) is a specialized tool designed for researchers studying Lamin A/C, a key component of the nuclear lamina involved in maintaining nuclear structure and function. This antibody, produced in rabbits, is highly specific to human samples and has been rigorously validated for use in immunohistochemistry and immunofluorescence applications.Lamin A/C is essential for proper cell function and integrity, with mutations in the LMNA gene associated with a range of diseases including muscular dystrophies and premature aging syndromes.
This antibody targets the Lamin A/C protein, allowing for precise detection and visualization in various cell types and tissues.Research into Lamin A/C is crucial for understanding its role in nuclear organization, gene regulation, and disease mechanisms. By utilizing the KO-validated Lamin A/C Rabbit Monoclonal Antibody (CAB19524), researchers can explore the functions of Lamin A/C in both normal and pathological conditions, providing valuable insights into cellular processes and potential therapeutic targets.
The protein encoded by this gene is part of the nuclear lamina, a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.
Purification Method:
Affinity purification
Gene ID:
4000
Clone Number:
ARC5001-08
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and Lamin A/C knockout (KO) HeLa cells, using [KO Validated] Lamin A/C Rabbit mAb (CAB19524) at 1:50000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
