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[KO Validated] Laminin beta 1 Rabbit Polyclonal Antibody (CAB19970)

SKU:
CAB19970
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
$167 - $503
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Description

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[KO Validated] Laminin beta 1 Rabbit Polyclonal Antibody (CAB19970)

The LAMB1 Polyclonal Antibody (CAB19970) is a valuable tool for researchers studying LAMB1, a key protein in the extracellular matrix that plays a crucial role in cell adhesion, migration, and signaling. This rabbit polyclonal antibody is validated for use in various applications, including Western blotting, immunohistochemistry, and ELISA, and is highly reactive with human samples.LAMB1 is known to be involved in processes such as tissue development, wound healing, and tumor metastasis, making it a target of interest in cancer research and regenerative medicine.

By targeting LAMB1 with this antibody, researchers can study its expression and localization in different cell types and tissues, providing valuable insights into its function and potential therapeutic implications.With its high specificity and sensitivity, the LAMB1 Polyclonal Antibody is a reliable tool for investigating the role of LAMB1 in various physiological and pathological processes, making it a valuable asset for scientists working in the fields of biology, medicine, and biotechnology.

Product Name:[KO Validated] Laminin beta 1 Rabbit Polyclonal Antibody
SKU:CAB19970
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1467-1786 of human Laminin beta 1 (NP_002282.2).
Sequence:EAKL RADE AKQS AEDI LLKT NATK EKMD KSNE ELRN LIKQ IRNF LTQD SADL DSIE AVAN EVLK MEMP STPQ QLQN LTED IRER VESL SQVE VILQ HSAA DIAR AEML LEEA KRAS KSAT DVKV TADM VKEA LEEA EKAQ VAAE KAIK QADE DIQG TQNL LTSI ESET AASE ETLF NASQ RISE LERN VEEL KRKA AQNS GEAE YIEK VVYT VKQS AEDV KKTL DGEL DEKY KKVE NLIA KKTE ESAD ARRK AEML QNEA KTLL AQAN SKLQ LLKD LERK YEDN QRYL EDKA QELA RLEG EVRS LLKD ISQK VAVY STCL
Tested Applications:WB ELISA
Recommended Dilution:WB,1:1000 - 1:4000
Synonyms:CLM; LIS5; 1
Positive Sample:HeLa
Conjugate:Unconjugated
Cellular Localization:Secreted, basement membrane, extracellular matrix, extracellular space.
Calculated MW:198kDa
Observed MW:230kDa

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the beta chain isoform laminin, beta 1. The beta 1 chain has 7 structurally distinct domains which it shares with other beta chain isomers. The C-terminal helical region containing domains I and II are separated by domain alpha, domains III and V contain several EGF-like repeats, and domains IV and VI have a globular conformation. Laminin, beta 1 is expressed in most tissues that produce basement membranes, and is one of the 3 chains constituting laminin 1, the first laminin isolated from Engelbreth-Holm-Swarm (EHS) tumor. A sequence in the beta 1 chain that is involved in cell attachment, chemotaxis, and binding to the laminin receptor was identified and shown to have the capacity to inhibit metastasis.

Purification Method:Affinity purification
Gene ID:3912
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from wild type (WT) and Laminin beta 1 knockout (KO) HeLa cells, using [KO Validated] Laminin beta 1 Rabbit pAb (CAB19970) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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