The KO-Validated L1CAM Polyclonal Antibody (CAB8555) is a critical tool for research involving L1CAM, a cell adhesion molecule that is implicated in various biological processes, including cell migration, adhesion, and signal transduction. This antibody, generated in rabbits, is highly specific and reactive with human samples, making it ideal for use in Western blot applications for the detection and analysis of L1CAM protein in different cell types.L1CAM, also known as Neural cell adhesion molecule L1, plays a crucial role in neuronal development and axon guidance. Dysregulation of L1CAM expression has been linked to various cancers, neurological disorders, and developmental abnormalities.
Therefore, studying the function of L1CAM is essential for understanding its role in disease pathogenesis and potential therapeutic targeting.By using the KO-Validated L1CAM Polyclonal Antibody, researchers can further investigate the molecular mechanisms underlying L1CAM-mediated biological processes and potentially identify novel diagnostic and therapeutic strategies for diseases associated with L1CAM dysregulation. This antibody is a valuable tool for studies in neuroscience, cancer biology, and developmental biology.
Product Name:
[KO Validated] L1CAM Rabbit Polyclonal Antibody
SKU:
CAB8555
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1143-1257 of human CD171/L1CAM (NP_000416.1).
Cell membrane, Cell projection, Single-pass type I membrane protein, growth cone.
Calculated MW:
140kDa
Observed MW:
85kDa/200-250kDa
The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons.
Purification Method:
Affinity purification
Gene ID:
3897
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CD171/L1CAM antibody (CAB8555) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
