The KRT20 Polyclonal Antibody (CAB17997) is a valuable tool for researchers studying KRT20, a type I keratin protein found in epithelial cells, especially in the gastrointestinal tract and other tissues. This rabbit polyclonal antibody is highly reactive with human samples and has been validated for use in various applications, including Western blot and immunohistochemistry.KRT20 is a marker for intestinal epithelial cells and is frequently used in cancer research to identify tumor cells originating from epithelial tissues.
Its expression pattern can provide valuable insights into the differentiation and proliferation of epithelial cells in both normal and disease states. The KRT20 Polyclonal Antibody allows for precise detection and analysis of KRT20 protein levels in different cell types, making it an essential tool for studies in cancer biology, cell biology, and tissue differentiation.
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21.
Purification Method:
Affinity purification
Gene ID:
54474
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and Cytokeratin 20 (Cytokeratin 20 (KRT20)) knockout (KO) HeLa cells, using [KO Validated] Cytokeratin 20 (KRT20) Rabbit pAb (CAB17997) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
