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[KO Validated] Cytokeratin 20 (KRT20) Rabbit Polyclonal Antibody (CAB17997)

SKU:
CAB17997
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KO Validated] Cytokeratin 20 (KRT20) Rabbit Polyclonal Antibody
SKU:CAB17997
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 245-424 of human Cytokeratin 20 (Cytokeratin 20 (KRT20)) (NP_061883.1).
Sequence:KYEV MAQK NLQE AKEQ FERQ TAVL QQQV TVNT EELK GTEV QLTE LRRT SQSL EIEL QSHL SMKE SLEH TLEE TKAR YSSQ LANL QSLL SSLE AQLM QIRS NMER QNNE YHIL LDIK TRLE QEIA TYRR LLEG EDVK TTEY QLST LEER DIKK TRKI KTVV QEVV DGKV VSSE VKEV EENI
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200
Synonyms:K20; CD20; CK20; CK-20; KRT21; 0)
Positive Sample:HeLa
Conjugate:Unconjugated
Cellular Localization:Cytoplasm.
Calculated MW:48kDa
Observed MW:48kDa

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21.

Purification Method:Affinity purification
Gene ID:54474
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from wild type (WT) and Cytokeratin 20 (Cytokeratin 20 (KRT20)) knockout (KO) HeLa cells, using [KO Validated] Cytokeratin 20 (KRT20) Rabbit pAb (CAB17997) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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