The KLF4 Polyclonal Antibody (CAB18088) is a valuable tool for researchers studying the role of KLF4, a transcription factor known to be involved in various cellular processes, including cell proliferation, differentiation, and stem cell self-renewal. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in Western blot and immunohistochemistry applications.KLF4, also known as Kruppel-like factor 4, has been found to be dysregulated in various diseases, including cancer, cardiovascular disease, and neurodegenerative disorders.
By targeting KLF4 with this antibody, researchers can gain insights into its function and potential therapeutic implications in these disease contexts.This antibody enables the detection and analysis of KLF4 protein expression in different cell types, making it an essential tool for studies in stem cell biology, cancer research, and developmental biology. Its high specificity and reactivity make it a reliable choice for researchers looking to unravel the complexities of KLF4 signaling pathways and its implications in human health and disease.
Product Name:
[KO Validated] KLF4 Rabbit Polyclonal Antibody
SKU:
CAB18088
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 180-479 of human KLF4 (NP_004226.3).
This gene encodes a protein that belongs to the Kruppel family of transcription factors. The encoded zinc finger protein is required for normal development of the barrier function of skin. The encoded protein is thought to control the G1-to-S transition of the cell cycle following DNA damage by mediating the tumor suppressor gene p53. Mice lacking this gene have a normal appearance but lose weight rapidly, and die shortly after birth due to fluid evaporation resulting from compromised epidermal barrier function. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
9314
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and KLF4 knockout (KO) HeLa cells, using [KO Validated] KLF4 Rabbit pAb (CAB18088) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
