The KO-validated KDM1A Polyclonal Antibody (CAB1156) is a crucial tool for researchers studying KDM1A, a key regulator of gene expression and chromatin remodeling. This polyclonal antibody, generated in rabbits, is highly specific and can reliably detect KDM1A in human samples. It has been rigorously validated for use in Western blotting applications, enabling precise and accurate analysis of KDM1A expression levels in various cell types.KDM1A, also known as lysine-specific histone demethylase 1A, plays a vital role in epigenetic regulation by removing methyl groups from histone proteins.
Dysregulation of KDM1A has been implicated in numerous diseases, including cancer and neurological disorders, making it a promising target for therapeutic intervention. By using the KO-validated KDM1A Polyclonal Antibody, researchers can gain valuable insights into the function of KDM1A and its potential implications for disease pathology and treatment strategies.
Antibody Name:
Anti-KDM1 Antibody [KO Validated]
Antibody SKU:
CAB1156
Antibody Size:
20uL, 50uL, 100uL
Application:
WB IF IP
Reactivity:
Human, Mouse, Rat
Host Species:
Rabbit
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 130-380 of human KDM1 (NP_055828.2).
Application:
WB IF IP
Recommended Dilution:
WB 1:500 - 1:2000 IF 1:50 - 1:100 IP 1:50 - 1:100
Reactivity:
Human, Mouse, Rat
Positive Samples:
MCF7
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 130-380 of human KDM1 (NP_055828.2).
Purification Method:
Affinity purification
Storage Buffer:
Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants.
BRAF35-HDAC complex protein BHC110; Flavin-containing amine oxidase domain-containing protein 2
Protein Family:
Lysine-specific histone demethylase
UniProt Gene Name:
KDM1AÂ Â
UniProt Entry Name:
KDM1A_HUMAN
Western blot analysis of extracts of MCF7 cells, using KDM1 antibody (CAB1156) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020).
Western blot analysis of extracts from normal (control) and KDM1 knockout (KO) HeLa cells, using KDM1 antibody (CAB1156) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Immunofluorescence analysis of A549 cells using KDM1 antibody (CAB1156).
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug KDM1 antibody (CAB1156). Western blot was performed from the immunoprecipitate using KDM1 antibody (CAB1156) at a dilition of 1:500.
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